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beta Defensin 3 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:400 dilution, for 1.5 hours at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:400 dilution, for 1.5 hours at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:100 dilution, for 1.5 hours at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:100 dilution, for 1.5 hours at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.
Blank control cells (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3 FITC Conjugated antibody (bs-7378R-FITC; green), Dilution: 1\u03bcg in 100 \u03bcL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG FITC Conjugated (orange), used under the same conditions.
Blank control cells (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3 FITC Conjugated antibody (bs-7378R-FITC; green), Dilution: 1\u03bcg in 100 \u03bcL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG FITC Conjugated (orange), used under the same conditions.

Applications

  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human
Overview
Catalog # bs-7378R
Product Name beta Defensin 3 Polyclonal Antibody
Applications ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human beta Defensin 3
Immunogen Range 23-67/67
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 414325
Swiss Prot P81534
Subcellular location Secreted
Synonyms BD 3; BD-3; BD3; beta 103; Beta defensin 103A; Beta defensin 103A precursor; Beta defensin 3; Beta-defensin 103; Beta-defensin 3; D103A_HUMAN; DEF B3; DEFB 103; DEFB 103A; DEFB 3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; Defensin; Defensin beta 103A; Defensin beta 3; Defensin like protein; Defensin-like protein; HBD 3; hBD-3; HBD3; HBP 3; HBP3; mBD3.
Background Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

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