beta Defensin 3 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7378R-Cy5-100uL |
| Product Name | beta Defensin 3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human beta Defensin 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 414325 |
| Swiss Prot | P81534 |
| Subcellular location | Secreted |
| Synonyms | BD 3; BD-3; BD3; beta 103; Beta defensin 103A; Beta defensin 103A precursor; Beta defensin 3; Beta-defensin 103; Beta-defensin 3; D103A_HUMAN; DEF B3; DEFB 103; DEFB 103A; DEFB 3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; Defensin; Defensin beta 103A; Defensin beta 3; Defensin like protein; Defensin-like protein; HBD 3; hBD-3; HBD3; HBP 3; HBP3; mBD3. |
| Background | Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is beta Defensin 3 Polyclonal Antibody, Cy5 Conjugated (bs-7378R-Cy5) validated for, and at what dilution?
bs-7378R-Cy5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does beta Defensin 3 Polyclonal Antibody, Cy5 Conjugated (bs-7378R-Cy5) react with?
Tested reactivity: Human.
How should bs-7378R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta Defensin 3 Polyclonal Antibody, Cy5 Conjugated (bs-7378R-Cy5)?
KLH conjugated synthetic peptide derived from human beta Defensin 3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7378R-Cy5)?
Cy5 on bs-7378R-Cy5: excitation / emission 625,650nm/670nm.