Histone H4 (tri methyl K20) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7403R-BF555 |
| Product Name | Histone H4 (tri methyl K20) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised methylpeptide derived from human Histone H4 around the methylation site of tri methyl K20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 121504 |
| Swiss Prot | P62805 |
| Synonyms | H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.Histone H3/l. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Histone H4 (tri methyl K20) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-7403R-BF555) validated for, and at what dilution?
bs-7403R-BF555 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H4 (tri methyl K20) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-7403R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-7403R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H4 (tri methyl K20) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-7403R-BF555)?
KLH conjugated Synthesised methylpeptide derived from human Histone H4 around the methylation site of tri methyl K20
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-7403R-BF555)?
AbBy Fluor-555 on bs-7403R-BF555: excitation / emission 553nm/568nm.