LSD2 / AOF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7415R-PerCP-Cy5.5-100uL |
| Product Name | LSD2 / AOF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LSD2 / AOF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 221656 |
| Swiss Prot | Q8NB78 |
| Subcellular location | Nucleus |
| Synonyms | LSD2 / AOF1; KDM1B; amine oxidase (flavin containing) domain 1; amine oxidase, flavin containing 1; AOF1; bA204B7.3; C6orf193; dJ298J15.2; Flavin-containing amine oxidase domain-containing protein 1; FLJ33898; FLJ34109; FLJ43328; KDM1B; KDM1B_HUMAN; LSD2; lysine (K)-specific demethylase 1B; Lysine-specific demethylase 2; Lysine-specific histone demethylase 1B; Lysine-specific histone demethylase 2. |
| Background | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LSD2 / AOF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-7415R-PERCP-CY5-5) validated for, and at what dilution?
BS-7415R-PERCP-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LSD2 / AOF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-7415R-PERCP-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should BS-7415R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LSD2 / AOF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-7415R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human LSD2 / AOF1
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-7415R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-7415R-PERCP-CY5-5: excitation / emission 482nm/694nm.