ZDHHC20 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7418R-Cy7 |
| Product Name | ZDHHC20 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DHHC-20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 253832 |
| Subcellular location | Cell membrane |
| Synonyms | DHHC-20; Probable palmitoyltransferase ZDHHC20; ZDH20CHUMAN; ZDHHC20; Zinc finger DHHC domain-containing protein 20. |
| Background | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZDHHC20 (zinc finger, DHHC-type containing 20) is a 365 amino acid multi-pass membrane protein that contains one DHHC-type zinc finger and is thought to function as a palmitoyltransferase, catalyzing the transformation of palmitoyl-CoA and a cysteine-conjugated protein to an S-palmitoyl protein and free CoA. In response to DNA damage, ZDHHC20, which exists as multiple alternatively spliced isoforms, may be phosphorylated by ATM or ATR. The gene encoding ZDHHC20 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZDHHC20 Polyclonal Antibody, Cy7 Conjugated (bs-7418R-Cy7) validated for, and at what dilution?
bs-7418R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZDHHC20 Polyclonal Antibody, Cy7 Conjugated (bs-7418R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Sheep, Horse, Rabbit.
How should bs-7418R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZDHHC20 Polyclonal Antibody, Cy7 Conjugated (bs-7418R-Cy7)?
KLH conjugated synthetic peptide derived from human DHHC-20
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-7418R-Cy7)?
Cy7 on bs-7418R-Cy7: excitation / emission 743nm/767nm.