Histone H3 (acetyl K27) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7451R-FITC |
| Product Name | Histone H3 (acetyl K27) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated Synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K27 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | acetyl H3; Histone H3(acetyl k27); H3K27; H 3; H3 3 Like Sequence MH921; H3 3A; H3; H3 Histone; H3 Histone Family Member E Pseudogene; H3.4; H3/A; H3/g; H31_HUMAN; H3F3; H3FA; H3FT; H3t; HIST1H3J; HIST3H3; Histone cluster 1, H3a; Histone H3 3 Pseudogene; Histone H3.1; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Histone H3 (acetyl K27) Polyclonal Antibody, FITC Conjugated (bs-7451R-FITC) validated for, and at what dilution?
bs-7451R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (acetyl K27) Polyclonal Antibody, FITC Conjugated (bs-7451R-FITC) react with?
Tested reactivity: Human, Rat.
How should bs-7451R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (acetyl K27) Polyclonal Antibody, FITC Conjugated (bs-7451R-FITC)?
KLH conjugated Synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K27
What are the excitation and emission wavelengths of the FITC conjugate (bs-7451R-FITC)?
FITC on bs-7451R-FITC: excitation / emission 494nm/518nm.