phospho-Smad2 (Ser250) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7464R-Cy3 |
| Product Name | phospho-Smad2 (Ser250) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser250 |
| Modification Site | Ser250 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4087 |
| Swiss Prot | Q15796 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Smad2 (phospho S250); p-Smad2 (phospho S250); hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3. |
| Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-Smad2 (Ser250) Polyclonal Antibody, Cy3 Conjugated (bs-7464R-Cy3) validated for, and at what dilution?
bs-7464R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Smad2 (Ser250) Polyclonal Antibody, Cy3 Conjugated (bs-7464R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig.
How should bs-7464R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Smad2 (Ser250) Polyclonal Antibody, Cy3 Conjugated (bs-7464R-Cy3)?
KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser250
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7464R-Cy3)?
Cy3 on bs-7464R-Cy3: excitation / emission 512,550nm/570,615nm.