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PAK3 (Ser154) Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-7481r-cy3
Product Name PAK3 (Ser154) Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human PAK3 around the phosphorylation site of Ser154
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5063
Swiss Prot O75914
Subcellular location Cytoplasm, Nucleus
Synonyms PAK3 (phospho-S154); p-PAK3; phospho-PAK3; PAK3 (phospho-Ser154); ARA; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; XLID30; bPAK; beta-PAK; Pak65alpha; Pak65beta; Stk4; PAK3_HUMAN; PAK3; Oligophrenin-3; p21-activated kinase 3 (PAK-3); 2.7.11.1; PAK3_MOUSE; CDC42/RAC effector kinase PAK-B; Pakb; PAK3_RAT; p65-PAK;
Application Dilution
WB =1:500-2000
IF IF=1:100-500

Frequently asked questions

Which applications is PAK3 (Ser154) Polyclonal Antibody, Cy3 Conjugated (bs-7481R-Cy3) validated for, and at what dilution?

bs-7481R-Cy3 is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PAK3 (Ser154) Polyclonal Antibody, Cy3 Conjugated (bs-7481R-Cy3) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse, Rabbit.

How should bs-7481R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PAK3 (Ser154) Polyclonal Antibody, Cy3 Conjugated (bs-7481R-Cy3)?

KLH conjugated Synthesised phosphopeptide derived from human PAK3 around the phosphorylation site of Ser154

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7481R-Cy3)?

Cy3 on bs-7481R-Cy3: excitation / emission 512,550nm/570,615nm.