SPON1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7520R-Cy7 |
| Product Name | SPON1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPON1/F spondin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10418 |
| Swiss Prot | Q9HCB6 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | f-spondin; VSGP/F-spondin; Spondin-1; Vascular smooth muscle cell growth-promoting factor; SPON1; KIAA0762; VSGP |
| Background | Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SPON1 Polyclonal Antibody, Cy7 Conjugated (bs-7520R-Cy7) validated for, and at what dilution?
bs-7520R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SPON1 Polyclonal Antibody, Cy7 Conjugated (bs-7520R-Cy7) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Rabbit.
How should bs-7520R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPON1 Polyclonal Antibody, Cy7 Conjugated (bs-7520R-Cy7)?
KLH conjugated synthetic peptide derived from human SPON1/F spondin
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-7520R-Cy7)?
Cy7 on bs-7520R-Cy7: excitation / emission 743nm/767nm.