phospho-Caveolin-2 (Tyr19) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7523R-Cy3 |
| Product Name | phospho-Caveolin-2 (Tyr19) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated syntheticphosphopeptide derived from mouse Caveolin2 around the phosphorylation site of Tyr19. |
| Modification Site | Tyr19 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 858 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Caveolin 2 phospho Y19; p-Caveolin 2phospho Y19; CAV; CAV2; CAV2_HUMAN; Caveolae protein 20 kD; Caveolin 2; Caveolin 2 isoform a and b; Caveolin 2 isoform c; Caveolin-2; MGC12294; CAV2_HUMAN. |
| Background | Cellular localization: Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Caveolin-2 (Tyr19) Polyclonal Antibody, Cy3 Conjugated (bs-7523R-Cy3) validated for, and at what dilution?
bs-7523R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Caveolin-2 (Tyr19) Polyclonal Antibody, Cy3 Conjugated (bs-7523R-Cy3) react with?
Tested reactivity: Mouse, Rat.
How should bs-7523R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Caveolin-2 (Tyr19) Polyclonal Antibody, Cy3 Conjugated (bs-7523R-Cy3)?
KLH conjugated syntheticphosphopeptide derived from mouse Caveolin2 around the phosphorylation site of Tyr19.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7523R-Cy3)?
Cy3 on bs-7523R-Cy3: excitation / emission 512,550nm/570,615nm.