BTG1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7526r-cy5 |
| Product Name | BTG1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BTG1 |
| Immunogen Range | 41-140/171 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 694, 7832 |
| Swiss Prot | P62324, P78543 |
| Subcellular location | Nucleus |
| Synonyms | B cell translocation gene 1 protein; B cell translocation gene 1, anti prolerative; BTG1 protein; BTG1_HUMAN; BTG2_HUMAN; Protein BTG2; BTG family member 2; NGF-inducible anti-prolerative protein PC3; pheochromacytoma cell-3; nerve growth factor-inducible anti-prolerative; B-cell translocation gene 2; BTG2; TIS21; PC3; TIS21 |
| Background | BTG1 belongs to the BTG family of proteins.FUNCTION: Anti-proliferative protein. Its expression is associated with the early G1 phase of the cell cycle. A chromosomal aberration involving BTG1 may be a cause of a form of B cell chronic lymphocytic leukemia; translocation t(8;12)(q24;q22) with MYC. BTG2 is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. BTG2 is involved in the regulation of the G1/S transition of the cell cycle. It modulates transcription regulation mediated by ESR1(referenced from Entrez Gene). BTG2 expression is induced in vivo during neurogenesis, and the gene is transiently expressed in vitro in rat pheochromocytoma PC12 cells after induction of neuronal differentiation by addition of nerve growth factor (NGF); suggesting that BTG2 is functionally significant during the neuronal differentiation process (PMID: 12360398). |
| Application Dilution | |
| IF | 1:100-500 |
Frequently asked questions
Which applications is BTG1 Polyclonal Antibody, Cy5 Conjugated (bs-7526R-Cy5) validated for, and at what dilution?
bs-7526R-Cy5 is validated for IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BTG1 Polyclonal Antibody, Cy5 Conjugated (bs-7526R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-7526R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BTG1 Polyclonal Antibody, Cy5 Conjugated (bs-7526R-Cy5)?
KLH conjugated synthetic peptide derived from human BTG1 Immunogen range: 41-140/171.
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7526R-Cy5)?
Cy5 on bs-7526R-Cy5: excitation / emission 625,650nm/670nm.