CPB2 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7547R-APC-100uL |
| Product Name | CPB2 Polyclonal Antibody, APC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CPB2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1361 |
| Subcellular location | Secreted |
| Synonyms | Carboxypeptidase B like protein; Carboxypeptidase U; CarboxypeptidaseB2; Carboxypeptidase B2; CarboxypeptidaseU; CP B2; CPB 2; CPB2; CPU; PCPB; Plasma carboxypeptidase B; Plasma carboxypeptidase B2; Procarboxypeptidase B; Procarboxypeptidase R; Procarboxypeptidase U; TAFI; Thrombin activable fibrinolysis inhibitor; Thrombin activatable fibrinolysis inhibitor. |
| Background | This protein belongs to a family of Zn-containing metallocarboxypeptidases specific to C-terminal lysine and arginine residues. It circulates in plasma as a zymogen with molecular weight of 55 kDa (401 amino acid residues; pI 5.0). Being activated by thrombin-thrombomodulin complex during blood coagulation, it exerts carboxypeptidase activity. Activated carboxypeptidase B2 removes C-terminal lysine residues from fibrin, which is necessary for plasminogen binding to fibrin. This prevents plasminogen from activation into plasmin and retards the lysis of a fibrin clot. The concentration in plasma of healthy people is 5-10 ug/ml. High plasma levels were found in patients with stable angina pectoris and angiographically verified coronary artery disease. Elevated concentration in blood is considered as a risk factor for venous thrombosis. A deficiency might contribute to the severity of bleeding disorders in hemophilias A and B, and decreased levels are found in chronic liver disease. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CPB2 Polyclonal Antibody, APC Conjugated (bs-7547R-APC) validated for, and at what dilution?
bs-7547R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CPB2 Polyclonal Antibody, APC Conjugated (bs-7547R-APC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse.
How should bs-7547R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CPB2 Polyclonal Antibody, APC Conjugated (bs-7547R-APC)?
KLH conjugated synthetic peptide derived from human CPB2
What are the excitation and emission wavelengths of the APC conjugate (bs-7547R-APC)?
APC on bs-7547R-APC: excitation / emission 650nm/660nm.