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TNFAIP3 interacting protein 3 Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Sheep
Overview
Catalog # bs-7551R-Cy3
Product Name TNFAIP3 interacting protein 3 Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Sheep
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TNFAIP3 interacting protein 3/ABIN3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7128
Subcellular location Cytoplasm
Synonyms Listeria induced; A20-binding inhibitor of NF-kappa-B activation 3; ABIN-3; ABIN-3 beta; FLJ21162; LIND; Listeria induced gene protein; TNFAIP3 interacting protein 3; TNFAIP3-interacting protein 3 beta; TNIP3; TNIP3 beta; TNIP3_HUMAN.
Background ABIN-3 is a member of the A20-binding inhibitor of NF-kappaB activation (ABIN) protein family. Similar to the previously characterized human ABINs (ABIN-1 and ABIN-2), ABIN-3 can bind to A20 and inhibit NF-kappaB activation. In contrast, mouse ABIN-3 is incapable of inhibiting NF-kappaB activation by proinflammatory stimuli because the protein lacks a complete ABIN homology domain, which is required for the funcitonal activity of human ABIN-3.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TNFAIP3 interacting protein 3 Antibody, Cy3 Conjugated (bs-7551R-Cy3) validated for, and at what dilution?

bs-7551R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TNFAIP3 interacting protein 3 Antibody, Cy3 Conjugated (bs-7551R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep.

How should bs-7551R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TNFAIP3 interacting protein 3 Antibody, Cy3 Conjugated (bs-7551R-Cy3)?

KLH conjugated synthetic peptide derived from human TNFAIP3 interacting protein 3/ABIN3

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7551R-Cy3)?

Cy3 on bs-7551R-Cy3: excitation / emission 512,550nm/570,615nm.