phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7554R-Cy5.5-100uL |
| Product Name | phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Eph receptor A2 around the phosphorylation site of Tyr588 |
| Modification Site | Tyr588 (A3 Tyr596) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1969 |
| Subcellular location | Cell membrane |
| Synonyms | Eph receptor A2+A3 phospho Y588 + Y596 ECK; EPH Receptor A2; EPH Receptor A3; EPHA2; EPHA3; Ephrin type A receptor 2; Ephrin type A receptor 3; Epithelial cell kinase; ETK; ETK1; HEK; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor ETK1. |
| Background | Eph receptor A2 is the receptor for members of the ephrin-A family. It binds to ephrin-A1, -A3, -A4 and -A5. Eph receptor A3 is also a receptor for members of the ephrin-A family. It binds to ephrin-A2, -A3, -A4 and -A5 and is thought to play a role in lymphoid function. Eph receptor A4 is a receptor for members of the ephrin-A family. It binds to ephrin-A1, -A4 and -A5. It binds more poorly to ephrin-A2 and -A3. It may play a role in a signal transduction process involved in hindbrain pattern formation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) Polyclonal Antibody, Cy5.5 Conjugated (BS-7554R-CY5-5) validated for, and at what dilution?
BS-7554R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) Polyclonal Antibody, Cy5.5 Conjugated (BS-7554R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should BS-7554R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) Polyclonal Antibody, Cy5.5 Conjugated (BS-7554R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Eph receptor A2 around the phosphorylation site of Tyr588
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-7554R-CY5-5)?
Cy5.5 on BS-7554R-CY5-5: excitation / emission 675nm/694nm.