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TIRAP Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Rabbit
Overview
Catalog # bs-7561R-BF555-100uL
Product Name TIRAP Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Rabbit
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TIRAP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 114609
Subcellular location Cytoplasm
Synonyms Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2; TIRAP; MAL
Background Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TIRAP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-7561R-BF555) validated for, and at what dilution?

bs-7561R-BF555 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TIRAP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-7561R-BF555) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Rabbit.

How should bs-7561R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TIRAP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-7561R-BF555)?

KLH conjugated synthetic peptide derived from human TIRAP

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-7561R-BF555)?

AbBy Fluor-555 on bs-7561R-BF555: excitation / emission 553nm/568nm.