phospho-TIRAP (Tyr86) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7562R-Cy5 |
| Product Name | phospho-TIRAP (Tyr86) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic acetylpeptide derived from human TIRAP around phosphorylation site of Tyr86 [KD(p-Y)DV] |
| Modification Site | Tyr86 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 114609 |
| Swiss Prot | P58753 |
| Subcellular location | Cytoplasm |
| Synonyms | Mal; wyatt; BACTS1; MyD88-2; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; TIRAP |
| Background | Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-TIRAP (Tyr86) Polyclonal Antibody, Cy5 Conjugated (bs-7562R-Cy5) validated for, and at what dilution?
bs-7562R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-TIRAP (Tyr86) Polyclonal Antibody, Cy5 Conjugated (bs-7562R-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, GuineaPig.
How should bs-7562R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-TIRAP (Tyr86) Polyclonal Antibody, Cy5 Conjugated (bs-7562R-Cy5)?
KLH conjugated synthetic acetylpeptide derived from human TIRAP around phosphorylation site of Tyr86 [KD(p-Y)DV]
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7562R-Cy5)?
Cy5 on bs-7562R-Cy5: excitation / emission 625,650nm/670nm.