5(6)-FAM N-succinimidyl ester

Overview
Catalog #	bs-75994c-100mg
Product Name	5(6)-FAM N-succinimidyl ester
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 117548-22-8 Molecular formula: C25H15NO9 Molecular weight: 473.4 Purity: >98% (HPLC) Appearance: Yellow to orange powder. Solubility: Soluble in DMSO or dimethylformamide. InChiKey: STUDUJDWFUAOEN-UHFFFAOYSA-N SMILES: OC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=O)=C1.CC(ON5C(CCC5=O)=O)=O
Description	Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492; lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0.