5-CFDA

Overview
Catalog #	bs-76007c-25mg
Product Name	5-CFDA
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at +4°C.
Target
Product Information	CAS Number: 79955-27-4 Molecular formula: C25H16O9 Molecular weight: 460.39 Purity: >95% (HPLC) Appearance: White to yellow solid. Solubility: Soluble in DMSO. InChiKey: WPUZGNPQMIWOHE-UHFFFAOYSA-N SMILES: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
Description	Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.