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5-CFDA N-succinimidyl ester

Overview
Catalog # bs-76028c-5mg
Product Name 5-CFDA N-succinimidyl ester
Specifications
Storage Buffer Powder
Storage Condition Stable for 2 years after receipt when stored at -20°C.
Target
Product Information CAS Number: 150206-05-6

Molecular formula: C29H19NO11

Molecular weight: 557.46

Purity: >95% (HPLC)

Appearance: White to faint yellow powder.

Solubility: Soluble in DMSO or DMF.

InChiKey: SRSXVRUMXPCNAJ-UHFFFAOYSA-N

SMILES: CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2
Description 5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.