Fluo-3 AM

Overview
Catalog #	bs-76303c-1mg
Product Name	Fluo-3 AM
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 121714-22-5 Molecular formula: C51H50Cl2N2O23 Molecular weight: 1129.85 Purity: >90% (TLC) Appearance: Dark red powder. Solubility: Soluble in DMSO. InChiKey: PTPUOMXKXCCSEN-UHFFFAOYSA-N SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C=C1OCCOC1=C(C=CC(C)=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C1=C2C=C(Cl)C(=O)C=C2OC2=CC(OCOC(C)=O)=C(Cl)C=C12 SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=C3C=C(Cl)C(=O)C=C3OC3=CC(O)=C(Cl)C=C23)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1
Description	Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.