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MTFR1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
Overview
Catalog # bs-7632R-Cy3
Product Name MTFR1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MTFR1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9650
Subcellular location Cytoplasm, Cell membrane
Synonyms MTFR 1; Chondrocyte protein with a poly proline region; Chondrocyte protein with a poly-proline region; CHPPR; FAM54A2; KIAA0009; Mitochondrial fission regulator 1; MTFR1_HUMAN.
Background This gene encodes a mitochondrial protein that is characterized by a poly-proline rich region. A chicken homolog of this protein promotes mitochondrial fission and the mouse homolog protects cells from oxidative stress. A related pseudogene of this gene is found on chromosome X.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MTFR1 Polyclonal Antibody, Cy3 Conjugated (bs-7632R-Cy3) validated for, and at what dilution?

bs-7632R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7632R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MTFR1 Polyclonal Antibody, Cy3 Conjugated (bs-7632R-Cy3)?

KLH conjugated synthetic peptide derived from human MTFR1

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7632R-Cy3)?

Cy3 on bs-7632R-Cy3: excitation / emission 512,550nm/570,615nm.