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SLC14A1 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-7639R-Cy5
Product Name SLC14A1 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SLC14A1/RACH1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms blood group Kidd urea transporter; HsT1341; HUT11; JK; Kidd; kidd JK blood group urea transporter B1; RACH1; solute carrier family 14 urea transporter member 1 Kidd blood group; urea transporter B1; Urea transporter erythrocyte;urea transporter JK glycoprotein; UT B1; UT1; UTE.
Background SLC14A1 is one of two major mammalian urea transporters, which play a critical role in the urine-concentrating mechanism. Their abundance is regulated by vasopressin, glucocorticoids, and mineralocorticoids. These regulatory mechanisms may be important in disease states such as diabetes.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SLC14A1 Polyclonal Antibody, Cy5 Conjugated (bs-7639R-Cy5) validated for, and at what dilution?

bs-7639R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SLC14A1 Polyclonal Antibody, Cy5 Conjugated (bs-7639R-Cy5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Chicken, Rabbit.

How should bs-7639R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SLC14A1 Polyclonal Antibody, Cy5 Conjugated (bs-7639R-Cy5)?

KLH conjugated synthetic peptide derived from human SLC14A1/RACH1

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7639R-Cy5)?

Cy5 on bs-7639R-Cy5: excitation / emission 625,650nm/670nm.