DNase II Polyclonal Antibody

$Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded.\\nAntigen retrieval: Citrate buffer ( 0.01M, pH 6.0 ) boiling bath for 15min; Blocking buffer (normal goat serum 10%) at 37\u2103 for 20 min;\\nIncubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4\u00b0C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue) was used to stain the cell nuclei\\n$

Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded.\\nAntigen retrieval: Citrate buffer ( 0.01M, pH 6.0 ) boiling bath for 15min; Blocking buffer (normal goat serum 10%) at 37\u2103 for 20 min;\\nIncubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4\u00b0C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue) was used to stain the cell nuclei\\n

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n$

Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n

$Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n$

Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Liver tissue lysates ; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Mouse Testis tissue lysates ; Lane 6: Rat Cerebrum tissue lysates ; Lane 7: Rat Liver tissue lysates ; Lane 8: Rat Kidney tissue lysates ; Lane 9: Human A431 cell lysates ; Lane 10: Human Huvec cell lysates probed with DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Liver tissue lysates ; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Mouse Testis tissue lysates ; Lane 6: Rat Cerebrum tissue lysates ; Lane 7: Rat Liver tissue lysates ; Lane 8: Rat Kidney tissue lysates ; Lane 9: Human A431 cell lysates ; Lane 10: Human Huvec cell lysates probed with DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Applications

ELISA
IHC-P
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-7652R
Product Name	DNase II Polyclonal Antibody
Applications	ELISA IHC-P IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DNase II alpha
Immunogen Range	25-90/360
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1777
Swiss Prot	O00115
Subcellular location	Cytoplasm
Synonyms	DNL; DNL2; DNASE2A; Deoxyribonuclease-2-alpha; Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II; R31240_2; DNASE2
Background	Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200