Resorufin benzyl ether
| Overview | |
| Catalog # | bs-76749c-5mg |
| Product Name | Resorufin benzyl ether |
| Specifications | |
| Storage Buffer | Powder |
| Storage Condition | Stable for 2 years after receipt when stored at -20°C. |
| Target | |
| Product Information | CAS Number: 87687-02-3 Molecular formula: C19H13NO3 Molecular weight: 303.31 Purity: >98% (HPLC) Appearance: Orange to red powder. Solubility: Soluble in DMSO. InChiKey: XNZRYTITWLGTJS-UHFFFAOYSA-N SMILES: O=C1C=C2OC3=CC(OCC4=CC=CC=C4)=CC=C3N=C2C=C1 SMILES: O=C1C=CC2=NC3=C(OC2=C1)C=C(OCC1=CC=CC=C1)C=C3 |
| Description | Resorufin benzyl ether (7-Benzyloxyresorufin) is a fluorogenic substrate widely used for monitoring cytochrome P450 (CYP450) activities in cell extracts and solutions. It is the model substrate for 7-Benzoxyresorufin-O-dealkylase (BROD) assays. It is typically used near its apparent Km value of 30 �M to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Cytochrome P450 oxidase is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site. Upon enzymatic cleavage of 7-Benzyloxyresorufin by CYP450, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=585nm. |
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