Corin Polyclonal Antibody

$Antigen: bs-7685P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-7685P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Formalin-fixed and paraffin embedded rat cardiac muscle labeled with Rabbit Anti Corin Polyclonal Antibody, Unconjugated (bs-7685R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Blank control:HepG2. \r\nPrimary Antibody (green line): Rabbit Anti-Corin antibody (bs-7685R) \r\nDilution: 1ug\/Test; \r\nSecondary Antibody : Goat anti-rabbit IgG-AF488\r\nDilution: 0.5ug\/Test. \r\nProtocol\r\nHela cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Corin Polyclonal Antibody(bs-7685R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

Blank control:HepG2. \r\nPrimary Antibody (green line): Rabbit Anti-Corin antibody (bs-7685R) \r\nDilution: 1ug\/Test; \r\nSecondary Antibody : Goat anti-rabbit IgG-AF488\r\nDilution: 0.5ug\/Test. \r\nProtocol\r\nHela cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Corin Polyclonal Antibody(bs-7685R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

ELISA
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Pig
Horse

Overview
Catalog #	bs-7685R
Product Name	Corin Polyclonal Antibody
Applications	ELISA, IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Corin
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10699
Swiss Prot	Q9Y5Q5
Subcellular location	Secreted, Cell membrane
Synonyms	CRN; ATC2; Lrp4; PEE5; TMPRSS10; Atrial natriuretic peptide-converting enzyme; Corin; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme; Transmembrane protease serine 10
Background	Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Isoform 2: has weaker endopeptidase activity compared to isoform 1.
Application Dilution
ELISA	1:500-1000
IF(ICC)	1:50-200