SLC12A3 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7694R-Cy5 |
| Product Name | SLC12A3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SLC12A3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6559 |
| Swiss Prot | P55017 |
| Subcellular location | Cell membrane |
| Synonyms | NCC; TSC; NCCT; Solute carrier family 12 member 3; Na-Cl cotransporter; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter; SLC12A3 |
| Background | Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SLC12A3 Polyclonal Antibody, Cy5 Conjugated (bs-7694R-Cy5) validated for, and at what dilution?
bs-7694R-Cy5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SLC12A3 Polyclonal Antibody, Cy5 Conjugated (bs-7694R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Rabbit.
How should bs-7694R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SLC12A3 Polyclonal Antibody, Cy5 Conjugated (bs-7694R-Cy5)?
KLH conjugated synthetic peptide derived from human SLC12A3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7694R-Cy5)?
Cy5 on bs-7694R-Cy5: excitation / emission 625,650nm/670nm.