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Retinoic acid induced 17 Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-7709R-APC-100uL
Product Name Retinoic acid induced 17 Antibody, APC Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Retinoic acid induced 17
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 57178
Subcellular location Cytoplasm, Nucleus
Synonyms Zinc finger MIZ domain-containing protein 1; MIZ; PIAS like protein hZimp10; PIAS like protein on chromosome 10; PIAS like protein Zimp10; PIAS-like protein Zimp10; RAI 17; RAI17; RAI-17; Retinoic acid induced 17; Retinoic acid induced protein 17; Retinoic acid-induced protein 17; TRAFIP10; Zimp10; Zinc finger containing Miz1 PIAS like protein on chromosome 10; Zinc finger MIZ domain-containing protein 1; Zinc finger MIZ-type containing 1; Zmiz1; ZMIZ1_HUMAN; hZIMP10; KIAA1224.
Background Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.Tissue specificity:Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Retinoic acid induced 17 Antibody, APC Conjugated (bs-7709R-APC) validated for, and at what dilution?

bs-7709R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Retinoic acid induced 17 Antibody, APC Conjugated (bs-7709R-APC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-7709R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Retinoic acid induced 17 Antibody, APC Conjugated (bs-7709R-APC)?

KLH conjugated synthetic peptide derived from human Retinoic acid induced 17

What are the excitation and emission wavelengths of the APC conjugate (bs-7709R-APC)?

APC on bs-7709R-APC: excitation / emission 650nm/660nm.

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