DSCC1 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7720R-Cy3 |
| Product Name | DSCC1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DSCC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79075 |
| Subcellular location | Nucleus |
| Synonyms | DCC1; DCC1_HUMAN; Defective in sister chromatid cohesion 1 homolog; Defective in sister chromatid cohesion protein 1 homolog; DSCC1; hDCC1; MGC5528; Sister chromatid cohesion protein DCC1. |
| Background | Loads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DSCC1 Polyclonal Antibody, Cy3 Conjugated (bs-7720R-Cy3) validated for, and at what dilution?
bs-7720R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7720R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DSCC1 Polyclonal Antibody, Cy3 Conjugated (bs-7720R-Cy3)?
KLH conjugated synthetic peptide derived from human DSCC1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7720R-Cy3)?
Cy3 on bs-7720R-Cy3: excitation / emission 512,550nm/570,615nm.