phospho-REC8 (Ser251) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7726R-Cy7 |
| Product Name | phospho-REC8 (Ser251) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251 |
| Modification Site | Ser251 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56739 |
| Swiss Prot | Q8C5S7 |
| Subcellular location | Nucleus |
| Synonyms | mrec; Rec8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p; Rec8; Mei8 |
| Background | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-REC8 (Ser251) Polyclonal Antibody, Cy7 Conjugated (bs-7726R-Cy7) validated for, and at what dilution?
bs-7726R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-REC8 (Ser251) Polyclonal Antibody, Cy7 Conjugated (bs-7726R-Cy7) react with?
Tested reactivity: Mouse.
How should bs-7726R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-REC8 (Ser251) Polyclonal Antibody, Cy7 Conjugated (bs-7726R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-7726R-Cy7)?
Cy7 on bs-7726R-Cy7: excitation / emission 743nm/767nm.