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SMC2 Polyclonal Antibody

Lane 1: Molt-4 cell lysates; Lane 2: Siha cell lysates probed with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Molt-4 cell lysates; Lane 2: Siha cell lysates probed with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Jurkat cell lysates probed with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Jurkat cell lysates probed with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMC2 Polyclonal Antibody, Unconjugated (bs-7727R) at 1:200 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Horse
Overview
Catalog # bs-7727R
Product Name SMC2 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SMC2
Immunogen Range 401-500/1197
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10592
Swiss Prot O95347
Subcellular location Cytoplasm, Nucleus
Synonyms CAP E; CAPE; Chromosome associated protein E; Chromosome-associated protein E; FLJ10093; hCAP E; hCAP-E; hCAPE; PRO0324; SMC 2; SMC protein 2; SMC-2; SMC2; SMC2 L1; SMC2_HUMAN; SMC2L1; Structural maintenance of chromosomes SMC family member chromosome associated protein E; Structural maintenance of chromosomes 2; Structural maintenance of chromosomes 2 like 1; Structural maintenance of chromosomes 2 yeast like 1; Structural maintenance of chromosomes protein 2; XCAP E homolog; XCAP-E homolog; XCAPE homolog.
Background Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SMC2 Polyclonal Antibody (bs-7727R) validated for, and at what dilution?

bs-7727R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SMC2 Polyclonal Antibody (bs-7727R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.

How should bs-7727R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SMC2 Polyclonal Antibody (bs-7727R)?

KLH conjugated synthetic peptide derived from human SMC2 Immunogen range: 401-500/1197.

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