SMC5 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7729R-Cy3 |
| Product Name | SMC5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMC5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23137 |
| Subcellular location | Nucleus |
| Synonyms | hSMC 5; hSMC5; SMC 5 SMC 5 structural maintenance of chromosomes 5 like 1 SMC5 L1 SMC5 protein SMC5 structural maintenance of chromosomes 5 like 1 SMC5L1 Structural maintenance of chromosomes 5 like 1 Structural maintenance of chromosomes protein 5; SMC5_HUMAN. |
| Background | Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SMC5 Polyclonal Antibody, Cy3 Conjugated (bs-7729R-Cy3) validated for, and at what dilution?
bs-7729R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMC5 Polyclonal Antibody, Cy3 Conjugated (bs-7729R-Cy3) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Rabbit.
How should bs-7729R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMC5 Polyclonal Antibody, Cy3 Conjugated (bs-7729R-Cy3)?
KLH conjugated synthetic peptide derived from human SMC5
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7729R-Cy3)?
Cy3 on bs-7729R-Cy3: excitation / emission 512,550nm/570,615nm.