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SMC6 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-7730R-HRP
Product Name SMC6 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SMC6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 79677
Subcellular location Cytoplasm, Nucleus
Synonyms SMC6L1; SMC 6; SMC6; SMC6 protein; SMC6 structural maintenance of chromosomes 6 like 1; Structural maintenance of chromosomes 6; Structural maintenance of chromosomes 6 like 1; Structural maintenance of chromosomes protein 6.
Background SMC5/6 complex is recruited to nuclease induced double strand breaks (DSBs) and is required for the recruitment of cohesin to DSBs. The human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is SMC6 Polyclonal Antibody, HRP Conjugated (bs-7730R-HRP) validated for, and at what dilution?

bs-7730R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SMC6 Polyclonal Antibody, HRP Conjugated (bs-7730R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-7730R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SMC6 Polyclonal Antibody, HRP Conjugated (bs-7730R-HRP)?

KLH conjugated synthetic peptide derived from human SMC6