CHMP4B+CHMP4C Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7744R-BF750-100uL |
| Product Name | CHMP4B+CHMP4C Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CHMP4B/CHMP4C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 128866, 92421 |
| Swiss Prot | Q9H444, Q96CF2 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | CHMP4B: Charged multivesicular body protein 4b, Chromatin-modifying protein 4b, CHMP4b, SNF7 homolog associated with Alix 1, SNF7-2, hSnf7-2, Vacuolar protein sorting-associated protein 32-2, Vps32-2, hVps32-2 CHMP4C: Charged multivesicular body protein 4C, Chromatin-modifying protein 4C, CHMP4C, SNF7 homolog associated with Alix 3 SNF7-3, hSnf7-3, Vacuolar protein sorting-associated protein 32-3, Vps32-3, hVps32-3 |
| Background | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CHMP4B+CHMP4C Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7744R-BF750) validated for, and at what dilution?
bs-7744R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHMP4B+CHMP4C Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7744R-BF750) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Rabbit.
How should bs-7744R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHMP4B+CHMP4C Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-7744R-BF750)?
KLH conjugated synthetic peptide derived from human CHMP4B/CHMP4C
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-7744R-BF750)?
AbBy Fluor-750 on bs-7744R-BF750: excitation / emission 749nm/775nm.