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NIR2 Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-7763R-FITC
Product Name NIR2 Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NIR2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9600
Subcellular location Cytoplasm, Cell membrane
Synonyms membrane-associated 1; DRES9; Drosophila retinal degeneration B; Drosophila retinal degeneration B homolog; Membrane-associated phosphatidylinositol transfer protein 1; NIR 2; NIR-2; NIR2; Phosphatidylinositol transfer protein; phosphatidylinositol transfer protein membrane associated; phosphatidylinositol transfer protein membrane-associated; PITM1_HUMAN; PITPnm 1; PITPNM; Pitpnm1; PYK2 N terminal domain interacting receptor 2; PYK2 N-terminal domain-interacting receptor 2; RD 9; RD9; RDGB; RDGB1; RDGBA1; retinal degeneration B alpha 1.
Background Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NIR2 Polyclonal Antibody, FITC Conjugated (bs-7763R-FITC) validated for, and at what dilution?

bs-7763R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NIR2 Polyclonal Antibody, FITC Conjugated (bs-7763R-FITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-7763R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NIR2 Polyclonal Antibody, FITC Conjugated (bs-7763R-FITC)?

KLH conjugated synthetic peptide derived from human NIR2

What are the excitation and emission wavelengths of the FITC conjugate (bs-7763R-FITC)?

FITC on bs-7763R-FITC: excitation / emission 494nm/518nm.