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SPECC1L Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-7773R-Cy5.5-100uL
Product Name SPECC1L Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPECC1L
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23384
Subcellular location Cytoplasm, Cell membrane
Synonyms Cytokinesis and spindle organization A; Cytospin-A; CYTSA; CYTSA_HUMAN; Renal carcinoma antigen NY-REN-22; SPECC1-like protein; specc1l; Sperm antigen with calponin homology and coiled-coil domains 1-like.
Background Involved in cytokinesis and spindle organization.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SPECC1L Polyclonal Antibody, Cy5.5 Conjugated (BS-7773R-CY5-5) validated for, and at what dilution?

BS-7773R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPECC1L Polyclonal Antibody, Cy5.5 Conjugated (BS-7773R-CY5-5) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should BS-7773R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPECC1L Polyclonal Antibody, Cy5.5 Conjugated (BS-7773R-CY5-5)?

KLH conjugated synthetic peptide derived from human SPECC1L

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-7773R-CY5-5)?

Cy5.5 on BS-7773R-CY5-5: excitation / emission 675nm/694nm.