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NEDD1 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Rabbit
Overview
Catalog # bs-7813R-Cy5
Product Name NEDD1 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NEDD1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 121441
Subcellular location Cytoplasm
Synonyms GCP WD; Neural precursor cell expressed, developmentally down regulated gene 1; TUBGCP7; FLJ35902; NEDD1_HUMAN.
Background May play an important role during the embryonic development and differentiation of the central nervous system. Human NEDD1 localises to the centrosome and mitotic spindle. It binds to the components of the gamma-tubulin ring complex and target this complex to the centrosome and spindle.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NEDD1 Polyclonal Antibody, Cy5 Conjugated (bs-7813R-Cy5) validated for, and at what dilution?

bs-7813R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7813R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NEDD1 Polyclonal Antibody, Cy5 Conjugated (bs-7813R-Cy5)?

KLH conjugated synthetic peptide derived from human NEDD1

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7813R-Cy5)?

Cy5 on bs-7813R-Cy5: excitation / emission 625,650nm/670nm.