NEK1 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7814R-APC |
| Product Name | NEK1 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NEK1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4750 |
| Swiss Prot | Q96PY6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | SRPS2; SRTD6; SRPS2A; NY-REN-55; Serine/threonine-protein kinase Nek1; Never in mitosis A-related kinase 1; NimA-related protein kinase 1; Renal carcinoma antigen NY-REN-55; NEK1; KIAA1901 |
| Background | Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NEK1 Polyclonal Antibody, APC Conjugated (bs-7814R-APC) validated for, and at what dilution?
bs-7814R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NEK1 Polyclonal Antibody, APC Conjugated (bs-7814R-APC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Rabbit.
How should bs-7814R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEK1 Polyclonal Antibody, APC Conjugated (bs-7814R-APC)?
KLH conjugated synthetic peptide derived from human NEK1
What are the excitation and emission wavelengths of the APC conjugate (bs-7814R-APC)?
APC on bs-7814R-APC: excitation / emission 650nm/660nm.