NEK8 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7815R-Cy5 |
| Product Name | NEK8 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NEK8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 284086 |
| Subcellular location | Cytoplasm |
| Synonyms | JCK; NEK 8; NEK12A; NEK8; NEK8_HUMAN; Never in mitosis A-related kinase 8; NIMA-family kinase NEK8; NIMA-related kinase 12a; Nima-related protein kinase 12a; NimA-related protein kinase 8; Serine/threonine-protein kinase Nek8. |
| Background | Required for renal tubular integrity.Tissue specificity: Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NEK8 Polyclonal Antibody, Cy5 Conjugated (bs-7815R-Cy5) validated for, and at what dilution?
bs-7815R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NEK8 Polyclonal Antibody, Cy5 Conjugated (bs-7815R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Chicken.
How should bs-7815R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEK8 Polyclonal Antibody, Cy5 Conjugated (bs-7815R-Cy5)?
KLH conjugated synthetic peptide derived from human NEK8
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7815R-Cy5)?
Cy5 on bs-7815R-Cy5: excitation / emission 625,650nm/670nm.