NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7816R-Cy7 |
| Product Name | NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Dog, Sheep, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NSP5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 92521 |
| Subcellular location | Nucleus |
| Synonyms | Cytokinesis and spindle organization B; Cytospin B; FLJ36955; HCMOGT 1; NSP; NSP5; Nuclear structure protein 5; SPECC1; Spectrin domain with coiled coils 1; Sperm antigen HCMOGT 1; Sperm antigen with calponin homology and coiled coil domains 1; Structure protein NSP5a3a; Structure protein NSP5a3b; Structure protein NSP5b3a; Structure protein NSP5b3b; CYTSB_HUMAN. |
| Background | NSP type 5 alpha 3 alpha is a novel structure protein belonging to the cytospin-A family, that may play a role in nuclear function during cell division. It is also highly expressed in certain tumor cell lines, suggesting that it may serve as a good tumor marker. It was was cloned as part of a chromosomal translocation involving PDGFRB in a case of juvenile myelomonocytic leukemia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, Cy7 Conjugated (bs-7816R-Cy7) validated for, and at what dilution?
bs-7816R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, Cy7 Conjugated (bs-7816R-Cy7) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Sheep, Rabbit.
How should bs-7816R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, Cy7 Conjugated (bs-7816R-Cy7)?
KLH conjugated synthetic peptide derived from human NSP5
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-7816R-Cy7)?
Cy7 on bs-7816R-Cy7: excitation / emission 743nm/767nm.