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NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Sheep
  • Rabbit
Overview
Catalog # bs-7816R-HRP
Product Name NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Human, Dog, Sheep, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NSP5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 92521
Subcellular location Nucleus
Synonyms Cytokinesis and spindle organization B; Cytospin B; FLJ36955; HCMOGT 1; NSP; NSP5; Nuclear structure protein 5; SPECC1; Spectrin domain with coiled coils 1; Sperm antigen HCMOGT 1; Sperm antigen with calponin homology and coiled coil domains 1; Structure protein NSP5a3a; Structure protein NSP5a3b; Structure protein NSP5b3a; Structure protein NSP5b3b; CYTSB_HUMAN.
Background NSP type 5 alpha 3 alpha is a novel structure protein belonging to the cytospin-A family, that may play a role in nuclear function during cell division. It is also highly expressed in certain tumor cell lines, suggesting that it may serve as a good tumor marker. It was was cloned as part of a chromosomal translocation involving PDGFRB in a case of juvenile myelomonocytic leukemia.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, HRP Conjugated (bs-7816R-HRP) validated for, and at what dilution?

bs-7816R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, HRP Conjugated (bs-7816R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Sheep, Rabbit.

How should bs-7816R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NSP5/NSP 5 alpha 3 alpha Polyclonal Antibody, HRP Conjugated (bs-7816R-HRP)?

KLH conjugated synthetic peptide derived from human NSP5