NUBP1 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7817R-Cy3 |
| Product Name | NUBP1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NUBP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4682 |
| Subcellular location | Cytoplasm |
| Synonyms | Cytosolic Fe S cluster assembly factor NUBP1; Cytosolic Fe-S cluster assembly factor NUBP1; NBP 1; NBP; NBP1; nubp1; NUBP1_HUMAN; Nucleotide binding protein e.coli MinD like; Nucleotide binding protein 1 E.coli MinD like; Nucleotide binding protein 1 MinD homolog, E. coli; Nucleotide binding protein 1; Nucleotide-binding protein 1. |
| Background | Implicated in the regulation of centrosome duplication (By similarity). Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May bind and transfer 2 labile 4Fe-4S clusters to target apoproteins. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NUBP1 Polyclonal Antibody, Cy3 Conjugated (bs-7817R-Cy3) validated for, and at what dilution?
bs-7817R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7817R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NUBP1 Polyclonal Antibody, Cy3 Conjugated (bs-7817R-Cy3)?
KLH conjugated synthetic peptide derived from human NUBP1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7817R-Cy3)?
Cy3 on bs-7817R-Cy3: excitation / emission 512,550nm/570,615nm.