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CDCA8 Polyclonal Antibody, Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Horse
  • Bee
  • Rabbit
Overview
Catalog # bs-7834R-Cy7
Product Name CDCA8 Polyclonal Antibody, Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Horse, Bee, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Borealin/CDCA8
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55143
Subcellular location Cytoplasm, Nucleus
Synonyms BOR; BOREA_HUMAN; Borealin; CDCA8; Cell division cycle associated 8; Cell division cycle-associated protein 8; Dasra B; Dasra-B; DasraB; FLJ10468; FLJ12042; hDasra-B; MESRGP; OTTHUMP00000004514; Pluripotent embryonic stem cell-related gene 3 protein.
Background Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CDCA8 Polyclonal Antibody, Cy7 Conjugated (bs-7834R-Cy7) validated for, and at what dilution?

bs-7834R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDCA8 Polyclonal Antibody, Cy7 Conjugated (bs-7834R-Cy7) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Horse, Bee, Rabbit.

How should bs-7834R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDCA8 Polyclonal Antibody, Cy7 Conjugated (bs-7834R-Cy7)?

KLH conjugated synthetic peptide derived from human Borealin/CDCA8

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-7834R-Cy7)?

Cy7 on bs-7834R-Cy7: excitation / emission 743nm/767nm.