DCTN2 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7836R-FITC |
| Product Name | DCTN2 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Dynamitin/DCTN2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10540 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 50 kD dynein associated polypeptide; 50 kDa dynein associated polypeptide; 50 kDa dynein-associated polypeptide; DCTN-50; DCTN2; DCTN2_HUMAN; DCTN50; dynactin 2 p50; dynactin 2; dynactin complex 50 kD subunit; Dynactin complex 50 kDa subunit; Dynactin subunit 2; p50 dynamitin; RBP50. |
| Background | Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DCTN2 Polyclonal Antibody, FITC Conjugated (bs-7836R-FITC) validated for, and at what dilution?
bs-7836R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DCTN2 Polyclonal Antibody, FITC Conjugated (bs-7836R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-7836R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DCTN2 Polyclonal Antibody, FITC Conjugated (bs-7836R-FITC)?
KLH conjugated synthetic peptide derived from human Dynamitin/DCTN2
What are the excitation and emission wavelengths of the FITC conjugate (bs-7836R-FITC)?
FITC on bs-7836R-FITC: excitation / emission 494nm/518nm.