Mad2L2 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7840R-Cy3 |
| Product Name | Mad2L2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Mad2L2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10459 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Homolog of REV7 S cerevisiae; HREV7; MAD2 homolog; MAD2 like 2; MAD2 mitotic arrest deficient like 2; MAD2B; Mitotic Arrest Deficient 2 L2; Mitotic arrest deficient homolog like 2; Mitotic arrest deficient like 2 yeast; Mitotic arrest deficient yeast homolog; Mitotic spindle assembly checkpoint protein MAD2B; REV 7; REV7; Weakly similar to Mitotic MAD2 protein S cerevisiae. |
| Background | MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Mad2L2 Polyclonal Antibody, Cy3 Conjugated (bs-7840R-Cy3) validated for, and at what dilution?
bs-7840R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7840R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mad2L2 Polyclonal Antibody, Cy3 Conjugated (bs-7840R-Cy3)?
KLH conjugated synthetic peptide derived from human Mad2L2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7840R-Cy3)?
Cy3 on bs-7840R-Cy3: excitation / emission 512,550nm/570,615nm.