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NUDC Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-7841R-Cy3
Product Name NUDC Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NUDC
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10726
Subcellular location Cytoplasm, Nucleus
Synonyms HNUDC; MNUDC; MNUDC protein; NPD011; Nuclear distribution gene C A.nidulans homolog; Nuclear distribution gene C homolog; Nuclear distribution gene C homolog A. nidulans; Nuclear distribution protein C homolog; Nuclear migration protein nudC; nudC; NUDC_HUMAN; OTTHUMP00000004405; SIG 92; SIG92.
Background This gene encodes a nuclear distribution protein that plays an essential role in mitosis and cytokinesis. The encoded protein is involved in spindle formation during mitosis and in microtubule organization during cytokinesis. Pseudogenes of this gene are found on chromosome 2. [provided by RefSeq, Feb 2012].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NUDC Polyclonal Antibody, Cy3 Conjugated (bs-7841R-Cy3) validated for, and at what dilution?

bs-7841R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NUDC Polyclonal Antibody, Cy3 Conjugated (bs-7841R-Cy3) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Rabbit.

How should bs-7841R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NUDC Polyclonal Antibody, Cy3 Conjugated (bs-7841R-Cy3)?

KLH conjugated synthetic peptide derived from human NUDC

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7841R-Cy3)?

Cy3 on bs-7841R-Cy3: excitation / emission 512,550nm/570,615nm.