ZWINT Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7852R-APC-Cy7-100uL |
| Product Name | ZWINT Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZWINT |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11130 |
| Subcellular location | Nucleus |
| Synonyms | HZwint1; KNTC 2 AP; KNTC2AP; MGC 117174; MGC117174; ZW10 interacting protein 1; ZW10 interactor; ZWINT 1; ZWINT1; ZWINT-1; ZWINT_HUMAN. |
| Background | ZWINT is a component of the MIS12 complex, which is required for kinetochore function and spindle checkpoint activity. ZWINT interacts with ZW10 and targets it to the kinetochore at prometaphase. ZWINT is detectable on the kinetochore till late anaphase. Inhibition of ZWINT expression results in chromosome missegregation, spindle checkpoint failure and, ultimately, cell death upon cytokinesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZWINT Polyclonal Antibody, APC-Cy7 Conjugated (bs-7852R-APC-Cy7) validated for, and at what dilution?
bs-7852R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7852R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZWINT Polyclonal Antibody, APC-Cy7 Conjugated (bs-7852R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human ZWINT
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-7852R-APC-Cy7)?
APC-Cy7 on bs-7852R-APC-Cy7: excitation / emission 650nm/780nm.