FAM3C Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7862R-Cy3 |
| Product Name | FAM3C Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAM3C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10447 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | Fam3c; FAM3C_HUMAN; family with sequence similarity 3, member C; GS3786;ILEI; Interleukin-like EMT inducer; Predicted osteoblast protein; Protein FAM3C. |
| Background | ILEI is a 227 amino acid, ubiquitously expressed protein containing an amino-terminal signal peptide. Elevated levels of ILEI translation are observed in oncogenic, Ras-transformed mammary epithelial cells and epithelial to mesenchymal transition (Emt) as well as tumor growth and metastasis. Also, overexpression of ILEI results in loss of ZO-1, a protein involved in tight junctions, and expression of cytoplasmic E-cadherin, which has been shown to influence loss of polarity and invasiveness. Due to this evidence, it is suspected that ILEI cooperates with oncogenic Ras to cause TGF-independent Emt and its overexpression is correlated with the invasion, metastasis and survival in a variety of cancers. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FAM3C Polyclonal Antibody, Cy3 Conjugated (bs-7862R-Cy3) validated for, and at what dilution?
bs-7862R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAM3C Polyclonal Antibody, Cy3 Conjugated (bs-7862R-Cy3) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Chicken.
How should bs-7862R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAM3C Polyclonal Antibody, Cy3 Conjugated (bs-7862R-Cy3)?
KLH conjugated synthetic peptide derived from human FAM3C
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7862R-Cy3)?
Cy3 on bs-7862R-Cy3: excitation / emission 512,550nm/570,615nm.