AFF4 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-7886R-Cy5 |
| Product Name | AFF4 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AFF4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27125 |
| Subcellular location | Nucleus |
| Synonyms | Alf4; Laf4; MGC107469; AF4/FMR2 family member 4; AF5Q31; ALL1 fused gene from chromosome 5q31; HSPC092; Lymphoid nuclear protein related to AF4 like; Major CDK9 elongation factor associated protein; MCEF; RP23-226J16.7; AFF4_HUMAN. |
| Background | May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AFF4 Polyclonal Antibody, Cy5 Conjugated (bs-7886R-Cy5) validated for, and at what dilution?
bs-7886R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-7886R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AFF4 Polyclonal Antibody, Cy5 Conjugated (bs-7886R-Cy5)?
KLH conjugated synthetic peptide derived from human AFF4
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-7886R-Cy5)?
Cy5 on bs-7886R-Cy5: excitation / emission 625,650nm/670nm.