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Cyclin K Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
Overview
Catalog # bs-7887R-FITC
Product Name Cyclin K Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Chicken
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cyclin K/CCNK
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8812
Swiss Prot O75909
Subcellular location Nucleus
Synonyms CCNK; CCNK_HUMAN; CPR 4; CPR4; Cyclin K; Cyclin-K; CyclinK; MGC9113.
Background Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Cyclin K Polyclonal Antibody, FITC Conjugated (bs-7887R-FITC) validated for, and at what dilution?

bs-7887R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cyclin K Polyclonal Antibody, FITC Conjugated (bs-7887R-FITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Chicken.

How should bs-7887R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cyclin K Polyclonal Antibody, FITC Conjugated (bs-7887R-FITC)?

KLH conjugated synthetic peptide derived from human Cyclin K/CCNK

What are the excitation and emission wavelengths of the FITC conjugate (bs-7887R-FITC)?

FITC on bs-7887R-FITC: excitation / emission 494nm/518nm.