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CLK3 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-7908R-Cy3
Product Name CLK3 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CLK3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1198
Swiss Prot P49761
Subcellular location Cytoplasm, Nucleus
Synonyms CDC like kinase 3; CDC-like kinase 3; CLK3; CLK3_HUMAN; Dual specicity protein kinase CLK3.
Background Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CLK3 Polyclonal Antibody, Cy3 Conjugated (bs-7908R-Cy3) validated for, and at what dilution?

bs-7908R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CLK3 Polyclonal Antibody, Cy3 Conjugated (bs-7908R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken.

How should bs-7908R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CLK3 Polyclonal Antibody, Cy3 Conjugated (bs-7908R-Cy3)?

KLH conjugated synthetic peptide derived from human CLK3

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-7908R-Cy3)?

Cy3 on bs-7908R-Cy3: excitation / emission 512,550nm/570,615nm.